Plant Transcription Factor Database
Previous version: v3.0
Nicotiana tomentosiformis
HSF Family
Species TF ID Description
XP_009588171.1HSF family protein
XP_009588494.1HSF family protein
XP_009589845.1HSF family protein
XP_009590974.1HSF family protein
XP_009590975.1HSF family protein
XP_009590976.1HSF family protein
XP_009591257.1HSF family protein
XP_009592186.1HSF family protein
XP_009594956.1HSF family protein
XP_009596927.1HSF family protein
XP_009600492.1HSF family protein
XP_009603012.1HSF family protein
XP_009603015.1HSF family protein
XP_009603016.1HSF family protein
XP_009603017.1HSF family protein
XP_009606363.1HSF family protein
XP_009606739.1HSF family protein
XP_009611319.1HSF family protein
XP_009611948.1HSF family protein
XP_009612082.1HSF family protein
XP_009612736.1HSF family protein
XP_009612973.1HSF family protein
XP_009617028.1HSF family protein
XP_009619930.1HSF family protein
XP_009620920.1HSF family protein
XP_009621033.1HSF family protein
XP_009622755.1HSF family protein
XP_009623591.1HSF family protein
XP_009628776.1HSF family protein
XP_009629680.1HSF family protein
XP_009631618.1HSF family protein
XP_009631619.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645